13 Jan Instructions: Answer all ques
Total 75 points
Due at the beginning of class on 3/24/15
Name ________________________________
Signature _____________________________
Instructions: Answer all questions within this file (additional pages are acceptable if necessary). All answers
must be TYPED. You are free to work together, but please use your own words in answering the questions.
Turn in your own printed version of this file with answers embedded into the blank areas. All plots should be
done in Microsoft Excel and pasted into this document.
[SUBSTRATE] In mM
Assignment. A terrible plague of H3N2 influenza has begun to decimate the earths population. You convince
your boss at your pharmaceutical company to let you work on developing a new neuraminidase inhibitor to
combat this new strain. You make several hundred potential inhibitors and assay them with the neuraminidase
isolated from this new strain. Frustratingly, none of the compounds has the required Ki of less than 2 M to
effectively inhibit this enzyme. Finally, in desperation attempt, you decide to make one last compound. You
then assay this compound with the viral neuraminidase (which is used in the assay at 1 nM concentration).
After doing a complete study at various substrate and inhibitor concentrations, you arrive at the
following data. The numbers inside the box are the reaction rates measured in nM/s. This table is an embedded
MS excel file that can easily be copied into Microsoft Excel by cutting and pasting.
0.0083
0.0233
0.0383
0.0533
0.0683
0.0833
0.1665
0.4665
0.7664
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